Figure 7

EGF activation induces nuclear translocation of FABP7 in glioma cells. Immunofluorescent staining of FABP7 in SF763 glioma cells displayed both cytoplasmic and nuclear immunoreactivity (A), whereas FABP7 staining in the nuclei was increased after EGF treatment for 2 days (B). The scale of photomicrographs is the same as in Figure 1A. C, the sum of nuclear and cytoplasmic FABP7 fluorescence in SF763 cells in each of the 10 randomly chosen fields was calculated, and EGF treatment significantly increased total FABP7 fluorescence. D, the ratios of nuclear/cytoplasmic FABP7 in untreated SF763 glioma cells and cells treated with 50 ng/ml of EGF for 1 day were semi-quantitatively measured. For each group, ratios of 20 cells were calculated from each of the 10 randomly chosen fields (p < 0.001). E, control SF763 cells demonstrated no difference in migration in the presence of either FABP7-specific sense or antisense ODNs (*, p = 0.136), whereas migration of EGF-treated SF763 cells was reduced by more than one-third in the presence of antisense ODNs (**, p = 0.004). F, FABP7 polyclonal antibodies also inhibited migration of both control and EGF-treated SF763 cells (*, p=0.003; **, p=0.01). Error bars represented the standard errors of the mean.