Figure 2
From: Quantitative evaluation of RASSF1Amethylation in the non-lesional, regenerative and neoplastic liver
Upper panel: RASSF1A promoter region with CpG islands subjected to analysis; underlined is the sequence reported in the middle panel showing the methylation profile of RASSF1A detected by MSP in HCC and corresponding cirrhosis. Middle panel: Methylation profile of RASSF1A detected by MSP in HCC and corresponding cirrhosis of cases 14,16,18 and 37. DNASssI treated: positive control for methylation; M: molecular marker; Human Placental DNA: negative control for methylation; m: methylated; u: unmethylated. The illustrated sequence refers to a HCC (case n = 37) showing both methylated and unmethylated alleles. Lower panel: Methylation profile of NORE1A detected by MSP in HCC and corresponding cirrhosis of cases 14,16,18 and 37. DNASssI treated: positive control for methylation; M: molecular marker; Human Placental DNA: negative control for methylation; m: methylated; u: unmethylated. The illustrated sequence refers to a HCC (case n = 37) showing the exclusive presence of unmethylated alleles.