Figure 2

Principle of three and two-color fluorescence viability assay: Bcr-Abl positive BaF3 cells were cultivated in the presence of 1μM Imatinibfor 8 hours. (a) Following Imatinib treatment cells were incubated with TMRM (given color: red), SYTO^®63 (given color: blue), and Picogreen (given color: green) and visualized using a confocal microscope with a 63X objective. (b) Following Imatinib treatment cells were incubated in a PBS-BSA solution containing PI (given color: red) and SYTO^®63 (given color: blue) and visualized by confocal microscopy. (c) Comparison of cell death indices determined by Annexin V-FITC staining or TMRM/Picogreen staining in Imatinib treated cells by means of FACS analysis. (d) Comparison of cell death indices determined by PI/SYTO63 staining or TMRM/Picogreen staining. Cells were visualized by confocal microscopy with a 40× objective. 10 different areas with each at least 20 cells were counted. Values are means of the ratio PI⁺ cells or Picogreen⁺ cells to total cells ± SD from 10 different areas.