Figure 7
From: The monoclonal antibody SM5-1 recognizes a fibronectin variant which is widely expressed in melanoma
Alignment of nine positive clones' fragments. Alignment of nine positive clones' fragments from EcoR I and Xho I digestion and comparison to fibronectin. E0: EcoR I site of polylinker; E1: EcoR I site in fibronectin DNA sequence at position 4150; E2: EcoR I site in fibronectin DNA sequence at position 6680; X0: Xho I site of polylinker; X1: Xho I site in fibronectin DNA sequence at position 4062; X2: Xho I site in fibronectin DNA sequence at position 4122; X3: Xho I site in fibronectin DNA sequence at position 4918. All nine clones overlapped from the poly(A). The shortest clone 184 spanned the sequence from the poly(A) end to 4503 bp of fibronectin; the longest clone146 spanned from poly(A) end to 1998 bp of fibronectin.