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Figure 6 | BMC Cancer

Figure 6

From: The monoclonal antibody SM5-1 recognizes a fibronectin variant which is widely expressed in melanoma

Figure 6

Sequencing strategy of positively identified clones. The insert was first sequenced by the universal primer T7 (or M13 -20 primer) in one orientation and by the universal M13 reverse primer in the reverse orientation. Since the range of sequenced DNA was short (a few hundred base pairs), a sequence from near the end of this run was used to design a second primer, which primed the sequence from this region and moved another few hundred nucleotides further. From the end of this run the third primer was designed and the circle was repeated until the entire insert had been covered.

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