Immunohistochemical detection of PDK1pSer241 in breast cancer specimens. A, IHC for PDK1pSer241 in paraffin blocks of benign (C,D) and malignant (A,B) breast tissue. Staining was conducted with either nonimmune IgG (B,D) or a PDK1pSer241 antibody (A,C). B, Tissue microarray cores of low (+) expressing and high (+++) expressing breast cancers stained for PDK1pSer241. PDK1pSer241 was abundant in both the cytosol and nucleus of the carcinoma. C, A bar graph depicting the results in the tissue microarray analysis of 177 evaluable cores expressing either no (0), low (+1), medium (+2) or strong (+3) PDK1pSer241 staining of normal (normal), DCIS (DCIS), node negative (node (-)), node positive (node (+)) and metastatic (node distant) breast carcinomas.