Inhibition of PI3K reduces MMP expression in Comma/PDK1 cells. Cells were treated for 24 hr with 5–40 μM LY294002. A, Media was concentrated and assayed for MMP activity by zymography as described in Fig. 3. Bar graph represents a densitometric scan of the zymogram. B, Cells were lysed and analyzed by western blotting for levels of Akt, Akt1pS473 and β-actin. Bar graph represents a densitometric scan of the western blots normalized for β-actin levels.