Expression of PIG-2 in human tissues by Northern analyses and immunohistochemical studies. Northern blotting analyses were performed to determine the expression of PIG-2 in different human tissues. Normal 12 lane multiple tissue northern blot (A) or human cancer cell line multiple northern-blot purchased from Clontech (B) was probed with a radioactively labeled CC282 partial cDNA (upper panel) or human β-actin cDNA control probe provided by Clontech (lower panel). (C) Total RNAs were isolated from normal lung tissue, primary lung cancer and from NCI-H441, NCI-H157 and NCI-H2009 lung cancer cell lines. Blot was hybridized with the randomly primed [32P]-labeled PIG-2 partial cDNA probe (the CC282 fragment). Human β-actin cDNA was used as a control probe (lower panel). (D) Comparison of PIG-2 mRNA expression in human tumor tissues and their corresponding normal counterparts (upper panel). Total RNAs were extracted from fresh human normal and cancer tissues. The same blot was probed with β-actin as a loading control (lower panel). (E-I) Immunohistochemical staining for PIG-2 expression of human normal muscle (E), leiomyosarcoma (F), normal colon tissue (G), colon cancer (H), and pancrease cancer tissue (I), Original magnification, × 100.