Figure 1

Identification and expression level of PIG-2 . A) Comparison gene expression profiles by DDRT-PCR from total RNA isolated from normal cervical tissue, primary cervical cancer, cervical cancer tissue metastatic to lymph node and from CasKi and CUMC-6 cervical cancer cell lines. Differential display was carried out 5' arbitrary primer H-AP28 (5' -AAGCTTACGATGC-3') and 3' H-T₁₁C (5' -AAGCTTTTTTTTTTTC-3'). The PCR products were resolved by electrophoresis. CC282 is the name of the partial PIG-2 gene product. The arrow identifies the location relative to other PCR products. (B) Total RNAs were isolated from normal cervical tissue, primary cervical cancer, cervical cancer tissue metastatic to lymph node and from CasKi andCUMC-6 cervical cancer cell lines. Blot was hybridized with the randomly primed [³²P]-labeled PIG-2 partial cDNA probe (the CC282 fragment). Human β-actin cDNA was used as a control probe (lower panel).