Figure 1

Figure 1 shows the expression of proliferation and apoptotic markers in the various compartments of the prostate. The immunomarkers are shown in columns from left to right (Ki67, Mcm-2, a-Casp3, and Bcl-2) and the compartments are shown in rows from top to bottom (Normal, HGPIN and IGCA). Normal compartment showed predominant basal cell (green arrows) positivity for Ki67 and Mcm-2. A shift towards the luminal compartment (red arrows) is noted for these two proliferation markers in HGPIN. Activated Caspase-3 on the other hand, showed a progressive decrease in the luminal cell staining from normal through PIN to cancer. Bcl-2 did not show any differential trend across compartments. Note that the strongly positive basal cells (for Bcl-2) serve as positive internal controls for the assay.