Detection of BRD4-NUT chimeric transcript is shown in a-d. Total RNA was reverse transcribed and cDNA was used as a template in (a) PCR amplification with BR2276F and NUT1194R primer combination and (b) PCR amplification with BR2276F and NUT294R primers. M, 100 bp DNA ladder; S, patient with aggressive carcinoma; B, no RNA in the cDNA synthesis. (c) Partial sequence chromatogram showing the in-frame fusion of nt 2380 of BRD4 (accession number AF386649) with nt 172 of NUT (accession number AF482429). (d) Partial sequence chromatogram showing the out-of-frame fusion of nt 2380 of BRD4 with nt 78 of NUT. Arrow indicate the junction of the BRD4 and NUT genes.