Upregulation of survival- and anti-apoptotic molecules by DEX.(A) Capan-1 cells were treated with DEX (1 μM) for 24, 48 or 72 h as indicated. Cells were lysed, proteins were harvested and analyzed by Western blot analysis. (B) MIA-Pa-Ca2, SU8686, T3M4, Capan-2, AsPC1 and PANC-1 were treated as described in Fig. 1. 48 h later proteins were harvested. Western blot analysis using antibodies toward BAG-1 and HSP-70 were performed. The p50, p36 and p29 subunits of BAG-1 as well as the band of HSP-70 are indicated. ACTIN expression is a marker for equal conditions. Antibodies used are: MKP-1 (42 kD), SGK-1 (50-55 kD), X-IAP (57 kD), Bcl-2 (28 kD), BAG-1 (p50, p36 and p29 subunits) HSP-70 (70 kD) and ACTIN (44 kD). The experiments have been performed three times with similar outcome.