Figure 6
Biosynthesis of [ 14 C]-cholesterol in PC3 cells under ZOL treatments. Cells were treated with ZOL (20 μM) for 2, 22 or 46 hours and [14C]-mevalolactone (2.25 μCi/ml) was added to the medium for 2 hours more. Sterols were separated by thin layer chromatography (silica gel F/ethyl acetate). [14C]-Cholesterol was revealed and quantified by autoradiography with Phophorimager® (Molecular Dynamics).