Table 2 Antibody source and usage
Primary antibodies | Secondary antibodies |
|---|---|
Polyclonal goat anti-human REC8 (E-18):sc-15152 (Santa Cruz) | Rabbit anti-goat-Cy3 (Sigma) |
Polyclonal rabbit anti-mouse REC8 antibody (602; kindly donated by Dr. C. Heyting, Wageningen, NL [23]) | Goat anti-rabbit IgG-Texas red (Vector Laboratories Ltd., UK), |
CREST serum against human kinetochore proteins (Acris Antibodies, Hiddenhausen, Germany) | Mouse anti-human IgG-FITC (Dr. MS Cragg) |
Polyclonal rabbit anti-MOS (C237: sc-86; Santa Cruz Biotech;Wembley, UK) | Mouse anti-rabbit IgG-FITC (Sigma, Dorset, UK) |
Polyclonal rabbit anti-SCP3 antiserum (kindly donated by Dr. C. Heyting, Wageningen, NL [46]) | Goat anti-rabbit IgG-FITC (Vector Laboratories Ltd., UK), |