In vivo (A) and in vitro growth characteristics (B) of murine gastric carcinoma cell lines. Three mice each were injected with the indicated tumor cell doses. Tumor growth was quantified by two perpendicular measurements of the tumor diameter and calculation of the volume as described in the Materials and Methods section. To determine in vitro growth characteristics, cells were grown in 24-well plates starting with the indicated cell numbers. At different time points cells from triplicate wells were harvested and counted. Results are shown as mean +/- standard deviation (SD). Best fit curves as well as doubling times in days (shown in brackets) were calculated using the GraphPad software.