Figure 4

Cancer cell transversal of the in vitro blood-brain barrier model. 50,000 BMEC were plated on 6.5 mm ø transwells coated with collagen and 8.0 μm pore size in DMEM/F12/10%FCS/10%Nu serum. After 3 days the cells had grown to confluence and the medium was removed and replaced with DMEM/F12. After two more days 10,000 human SK-melanoma cells (SK-Mel) labeled with Cell-Tracker Orange™ were added. These co-cultures were further incubated in the absence of exogenous plasminogen (control), in the presence of 1 μg/ml plasminogen (plg) and in the presence of plasminogen and EACA (EACA). The results are the average of three experiments performed in duplicate. Error bars show standard deviation. *p < 0.01.