Table 1 Oligonucleotide sequences and conditions for PCR analyses
From: Cyclin A1 promoter hypermethylation in human papillomavirus-associated cervical cancer
Primer | Sequence | Amplicon size (bp) | Annealing temperature (°C) |
|---|---|---|---|
CCNA1metF | TTTCGAGGATTTCGCGTCGT | 46 | 53 |
CCNA1metR | CTCCTAAAAACCCTAACTCGA | Â | Â |
CCNA1unmetF | TTAGTGTGGGTAGGGTGTT | 67 | 53 |
CCNA1unmetR | CCCTAACTCAAAAAAACAACACA | Â | Â |
CCNA1cloningF | TGGGTAGGGCGTCGTAGTT | 196 | 55 |
CCNA1cloningR | GCCCCCGACCTAAAAAAA | Â | Â |
CCNA1cDNAF | ATTCATTAAGTGAAATTGTGC | 170 | 47 |
CCNA1cDNAR | CTTCCATTCAGAAACTTATTG | Â | Â |
GAPDHF | GTGGGCAAGGTATCCCTG | 460 | 52 |
GAPDHR | GATTCAGTGTGGTGGGGGAC | Â | Â |