Figure 3
From: Cyclin A1 promoter hypermethylation in human papillomavirus-associated cervical cancer
CCNA1 methylation and expression in microdissected cervical tissues. (A) Duplex MSP and CCNA1 PCR; E and CNT are epithelium and connective tissue cells from normal cervix; N and T are adjacent histological normal and cancer cervical epithelium from CC, respectively. Arrows indicate non-methylated, methylated, CCNA1 cDNA and GAPDH cDNA, respectively. (B) Bisulfite sequencing at the CCNA1 promoter, with circles denoting the methylation status of each selected clone. Black and white circles are methylated CG dinucleotides, and non-methylated CpG dinucleotides and TG dinucleotides, respectively.