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Figure 2 | BMC Cancer

Figure 2

From: Cyclin A1 promoter hypermethylation in human papillomavirus-associated cervical cancer

Figure 2

Intra- and inter-assay variation of the duplex MSP. (A) Duplex MSP of a mixture of CCNA1 complete and non-methylated CC cell lines, SiHa and HeLa(S), respectively. M, DNA size marker; Neg, negative; 0, 25, 50, 75, 100 Met (%) represent the proportion of SiHa DNA in the mixture, varied from 0 to 100%, respectively. The upper and lower bands are non-methylated and methylated bands, respectively, indicated by labeled arrows. (B) Graphical comparison between measured CCNA1 methylation, percentage intensity of methylation amplicon (x-axis), and actual methylation, the proportion of SiHa DNA (y-axis). The bar height indicates the mean and error bars, T, represent standard deviation (SD) across experiments.

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