Antitumor activities of cyclopamine and γ-secretase inhibitor against HEKTER-LUK and HEKTER-ST cell lines. (A) Survival of HEK-TERV and HEK-TERST cells determined by MTT assay after treatment with vehicle, 2.4μM Cyclopamine or 1 nM γ-secretase inhibitor for 72 hrs. Mean and standard error are shown for each treatment. Percent cell survival was computed relative to untreated cells. (B) Survival of HEK-TERST cells determined by Trypan Blue exclusion assay after treatment of 1 × 105 cells with vehicle, 2.4μM Cyclopamine or 1 nM γ-secretase inhibitor for 72 hrs.