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Figure 3 | BMC Cancer

Figure 3

From: Cross-platform expression profiling demonstrates that SV40 small tumor antigen activates Notch, Hedgehog, and Wnt signaling in human cells

Figure 3

(A) QRTPCR validation of the AB Expression Array System measured Fold Change of gene expression data between HEK-TERST and HEK-TERV cells. Fold change values from the AB platform are plotted on the x-axis and TaqMan assay based QRTPCR fold change is plotted on the y-axis. Data for the same set of genes are shown in all panels (A-F). (B) QRTPCR validation of the Affymetrix measured Fold Change of gene expression data between HEK-TERST and HEK-TERV cells. Fold change values from the Affymetrix platform are plotted on the x-axis and TaqMan assay based QRTPCR fold change is plotted on the y-axis. (C) Comparison of SYBR Green reagent based QRTPCR data to the AB Expression Array System fold change data. (D) Comparison of SYBR Green regent based QRTPCR data to the Affymetrix fold change data. (E) Comparison of the SYBR Green regent based QRTPCR data and the TaqMan assay based QRTPCR data. (F) Scatterplot comparison of the Affymetrix measured and AB Expression Array System measured Fold Change of gene expression data between HEK-TERST and HEK-TERV cells.

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