I) Representative examples of MSP for CDH1 gene in infiltrating ductal carcinomas. Lanes (m) and (u) correspond to reactions specific for methylated and unmethylated DNA, respectively. M, molecular weight DNA marker. II) Patterns of E-cadherin expression in ductal breast carcinomas stratified according a four-step scale: a. negative; b. 1+; c. 2+; and d. 3+. The sections were counterstained with hematoxylin. 400× magnification.