Table 3 Flow cytometry determination of the binding level of the scFv clone, M39, and its affinity-matured variant, E8, on living/intact human cells. A. Stained cells: -, (0–5 %); +/-, (6–25 %); +, (26–50 %); ++, 51–75 %); +++, (76–100 %); B. Melanoma cells derived from characterized specimens of tumor patients; C. PBMC and specific cell subpopulations derived from blood of healthy donors. No differences were observed in MA39 or E8 reactivity using cells from different donors.
From: Selection, affinity maturation, and characterization of a human scFv antibody against CEA protein
| Cell type | Tissues | MA39 | E8 | Notes |
|---|---|---|---|---|
| HT-29 | Colorectal adenocarcinoma | + | ++ | A |
| LoVo | Colorectal adenocarcinoma | + | ++ | |
| KB3.1 | Cervical squamous cell carcinoma | - | - | |
| MCF7 | Breast adenocarcinoma | +/- | + | |
| U937 | Monocytic lymphoma | - | - | |
| Mel.1 | Primary melanoma | +/- | + | B |
| Mel.2 | Primary melanoma | +/- | + | |
| Mel.3 | Metastatic melanoma | ++ | +++ | |
| Mel.4 | Metastatic melanoma | ++ | +++ | |
| CCFR-CEM | T cell acute lymphoblastic leukemia | - | - | |
| H69 | Small cell lung carcinoma | - | +/- | |
| Jurkat | T cell acute leukemia | - | - | |
| GLC4 | Small cell lung carcinoma | - | - | |
| K-562 | Chronic myelogenous leukemia | - | - | |
| RPMI8226 | B lymphocyte myeloma | - | - | |
| MV-4-11 | B myelomonocytic leukemia | - | - | |
| THP-1 | Acute monocytic leukemia | - | - | |
| HL-60 | Acute promyelocytic leukemia | - | - | |
| HF | Adult primary fibroblasts | - | - | |
| PBMC | total | - | - | C |
| CD4+ | - | - | ||
| CD14+ | - | - | ||
| Granulocytes | - | - | ||
| Neutrophils | - | - |