Table 3 Flow cytometry determination of the binding level of the scFv clone, M39, and its affinity-matured variant, E8, on living/intact human cells. A. Stained cells: -, (0–5 %); +/-, (6–25 %); +, (26–50 %); ++, 51–75 %); +++, (76–100 %); B. Melanoma cells derived from characterized specimens of tumor patients; C. PBMC and specific cell subpopulations derived from blood of healthy donors. No differences were observed in MA39 or E8 reactivity using cells from different donors.

From: Selection, affinity maturation, and characterization of a human scFv antibody against CEA protein