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Table 3 Flow cytometry determination of the binding level of the scFv clone, M39, and its affinity-matured variant, E8, on living/intact human cells. A. Stained cells: -, (0–5 %); +/-, (6–25 %); +, (26–50 %); ++, 51–75 %); +++, (76–100 %); B. Melanoma cells derived from characterized specimens of tumor patients; C. PBMC and specific cell subpopulations derived from blood of healthy donors. No differences were observed in MA39 or E8 reactivity using cells from different donors.

From: Selection, affinity maturation, and characterization of a human scFv antibody against CEA protein

Cell type Tissues MA39 E8 Notes
HT-29 Colorectal adenocarcinoma + ++ A
LoVo Colorectal adenocarcinoma + ++  
KB3.1 Cervical squamous cell carcinoma - -  
MCF7 Breast adenocarcinoma +/- +  
U937 Monocytic lymphoma - -  
Mel.1 Primary melanoma +/- + B
Mel.2 Primary melanoma +/- +  
Mel.3 Metastatic melanoma ++ +++  
Mel.4 Metastatic melanoma ++ +++  
CCFR-CEM T cell acute lymphoblastic leukemia - -  
H69 Small cell lung carcinoma - +/-  
Jurkat T cell acute leukemia - -  
GLC4 Small cell lung carcinoma - -  
K-562 Chronic myelogenous leukemia - -  
RPMI8226 B lymphocyte myeloma - -  
MV-4-11 B myelomonocytic leukemia - -  
THP-1 Acute monocytic leukemia - -  
HL-60 Acute promyelocytic leukemia - -  
HF Adult primary fibroblasts - -  
PBMC total - - C
CD4+   - -  
CD14+   - -  
Granulocytes   - -  
Neutrophils   - -