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Table 1 Oligonucleotide primers used for construction of mutant libraries for affinity maturation of anti-CEA MA39 scFv antibody. Wobble symbol N means G or A or T or C, symbol S means G or C. In underlined part of oligonucleotides KM144 and KM145, each base was replaced with mixture of G/A/T/C at a frequency of 10%.

From: Selection, affinity maturation, and characterization of a human scFv antibody against CEA protein

KM144 5'-TGTGCGAAAAGTAATGAGTTTCTTTTTGACTACTGGGGC-3'
KM145 5'-TCCGCCGAATACCACATAGGGCAACCACGGATAAGAGGAGTTACAGTAATAGTCAGCC-3'
KM149 5'-GTATATTACTGTGCGAAANNSNNSNNSAGTAATGAGTTTCTTNNSNNSNNSTTTGACTACTGGGGCCAG-3'
KM150 5'-GTATATTACTGTGCGAAANNSNNSAGTAATGAGTTTCTTNNSNNSNNSTTTGACTACTGGGGCCAG-3'
KM151 5'-GTATATTACTGTGCGAAANNSNNSNNSAGTAATGAGTTTCTTNNSNNSTTTGACTACTGGGGCCAG-3'
KM152 5'-GTATATTACTGTGCGAAANNSNNSAGTAATGAGTTTCTTNNSNNSTTTGACTACTGGGGCCAG-3'
KM148 5'-CTATTGCCTACGGCAGCCGCTGGA-3'
KM157 5'-TTTCGCACAGTAATATACGG-3'
KM158 5'-TATGTGGTATTCGGCGGA-3'
KM143 5'-GTCATCGTCGGAATCGTCATCTGC-3'
KM169 5'-GGTGGTAGCACATACTACGCA-3'
KM170 5'-GGAATTGTCTCTGGAGATGGTGA-3'
KM171 5'-TCGTCTGAGCTGACTCAGGAC-3'
KM172 5'-GCATGTGATCCTGACTGTCTG-3'
KM173 5'-CTCCAGCTCAGGAAACACAGCTT-3'
KM174 5'-AGCCTCATCTTCCGCCTGAG-3'
KM252 5'-GAGCTGGGTCCGCCAGGCTCCAGGGAAGGGGC-3'
KM253 5'-GAGCTGGGTCCGCCAGGCTCCAGGGAAGGGGC-3'