Figure 4From: Selection, affinity maturation, and characterization of a human scFv antibody against CEA protein ELISA reactivity of original and mutated anti-CEA antibodies. Equal quantity of the soluble scFv antibodies were assayed on CEA-coated plates. Bound scFvs were developed by using anti-FLAG secondary antibody. Results are expressed as A = A405-A620. Data reported are the average values of two independent assays.Back to article page