Figure 2
From: Selection, affinity maturation, and characterization of a human scFv antibody against CEA protein
Recognition of CEA by MA39 antibody. A. Immunodetection of CEA in LoVo cells by MA39 scFv (left slide). The same slide preparation incubated with irrelevant anti-glucose oxidase (a protein not present in mammals) scFv antibody (anti-G.O., right slide). B. Biochemical patterns of the MA39 on purified CEA (lane 1) and LoVo cellular extract (lane 2). Lanes 3 and 4 show the reactivity of identical proteins with irrelevant anti-G.O. scFv. C. Staining of cryosections from human colon carcinoma xenograft in mouse. Commercially available anti-CEA antibody and irrelevant anti-G.O. antibodies were used as positive and negative controls, respectively.