A transcriptome anatomy of human colorectal cancers

BMC Cancer

Table 1 Primers and amplification conditions of 8 selected putative genes

Gene Name	Primers	PCR Fragments (bp)	Annealing Temperature(C)	Cycles
TWISTNB	ATAACATCAAAGTTGTGGGAGA	196	48	35
	TCAGGTTTAGGAATGGAGGC
YPEL2	ATCGAAGACTTTCCAGGCATAT	344	57	36
	ATCAGTCCCAGCCATTGTCC
YPEL5	TAAACCTGGAGACTCGGGTGG	205	59	35
	AGCGGTTGGTCAGGATCGTAT
Sep6	AAGGTTGCCGAACTGTCC	280	59	36
	AAGCCAACTGTGCTAACGA
Sep4	CGAAAGTCCGTGAAGAAAG	221	59	34
	GTGTCCACAATGGTGAGCC
CBX3	ATAATTGGTGCCACAGACA	325	53	31
	AACAACTCTTCCAACGCTA
SOX9	AGGTGCTCAAAGGCTACGACT	360	63	32
	AGATGTGCGTCTGCTCCGTG
β-catenin	TCTTGGCTATTACGACAG	459	58	32
	CCTCTATACCACCCACTT

ISSN: 1471-2407