Conventional and molecular cytogenetic data of case 2 at diagnosis and recurrence. 4a: R-banded karyotype from initial tumor characterized by complex numerical and structural clonal abnormalities. Arrows indicate structural clonal aberrations. 4b: Partial R-banded details associated with partial Multiplex FISH results of each structural chromosomal abnormality. Painting chromosomes by M-FISH are showed in false color view with fluorescence profiles. 4c:Interphase in situ hybridization with a chromosome 8 centromeric probe directly labelled FITC from recurrent tumor showing over representation (10 signals) of centromeric region of chromosome 8 : nuc ish 8 cen (D8Z1x10).