Conventional and molecular cytogenetic data of case 1 at recurrence. 3a: R-banded karyotype: arrows indicate structural clonal abnormalities. 3b: Partial R-banded details associated with partial Multiplex FISH results of each structural chromosomal abnormality. Painting chromosomes by M-FISH are showed in false color view with fluorescence profiles. 3c: Partial metaphase in situ hybridization with a chromosome 8 centromeric probe directly labelled FITC showing one normal chromosome 8 (fine arrow) and one der(8) (thick arrow). 3d: Partial metaphase in situ hybridization with a TP53 probe directly labelled Spectrum Orange (SO) and a chromosome 17 centromeric probe directly labelled FITC. This partial metaphase shows one normal chromosome 17 with both FITC and SO fluorescent signals (fine arrow) and one der(17) with an isolated FITC fluorescent signal (thick arrow) indicating a P53 gene deletion.