The relative standard curve using the TGM3 gene, primers, and probes amplified with 100, 10, 1, 0.1 and 0.01 ng of total RNA. Each sample was run in triplicate (red square). The average CT values (y-axis) are plotted against the logarithm of the input amount of RNA (x-axis) added to each sample. PCR efficiency (E) = (10-1/slop - 1). It shows a linear relationship between RNA concentration and the CT value of reverse transcription real-time PCR reaction for TGM3.