Figure 8From: Nestin expression in the cell lines derived from glioblastoma multiforme Detection of nestin in the cell nucleus. Labeled nestin was distinctly detectable also in the cell nucleus as individual particles and small clusters of signal (A); several larger nestin aggregates (B–C) were also observed. Arrows indicate very short fibers in these aggregates (B–C). A special arrangement into short spiral-like fibers in cell nucleus (D–E) was found repeatedly in different cells. Nestin was detected using immunogold labeling; bars, 1 μm (A), 0.1 μm (B–E). Negative control stained with secondary antibody only: nucleus and nucleolus (Nu); bar, 0.5 μm (F).Back to article page