Figure 1

Nestin expression in primary cultures of glioblastoma cells. Nestin-positive intermediate filaments were detected in primary cultures in cells with different morphology – both in giant cells (A) and in smaller cells forming colonies (B). Due to heterogeneity of primary culture, non-tumor cells exhibiting no or very poor signals for nestin were also detectable together with nestin-positive cells (C). Nestin-positive filaments (A–C, green) stained by indirect immunofluorescence using FITC-labeled secondary antibody; nuclei labeled by DAPI (A–C, blue) are shown; bar, 25 μm.