Figure 4
From: Reduced repair of 8-hydroxyguanine in the human breast cancer cell line, HCC1937
Expression of specific DNA repair proteins. Total proteins (50 μg) were separated by SDS-PAGE and the proteins transferred onto a PVDF membrane. The immunoblots were probed with antibodies against the specific proteins. Panel A represents basal levels of OGG1, NEIL1, p53 and APE1 in AG11134, MCF-7 and HCC1937 and, panel B shows RT-PCR showing the levels of OGG1 1a mRNA transcripts in AG11134, HCC1937 and MCF-7 cells. β-actin was used as a loading control.