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Table 1 Antibodies

From: Tissue microarray analysis reveals a tight correlation between protein expression pattern and progression of esophageal squamous cell carcinoma

Antibody

Clone

Dilution &condition

Pretreatment

Source

Fas (C-20)

 

1:200 RT1 h

MWO-CB

Santa Cruz Bio, Santa Cruz, CA, USA

FADD

64A6

1:20 4°C overnight

MWO-CB

Novacastra, Newcastle upon Tyne, UK

Caspase 8

8CSP01

1:200 4°C overnight

MWO-CB

NeoMarkers, Fremont, CA, USA

CDC25B

 

1:50 RT1 h

MWO-CB

Cell Signaling, Beverly, MA, USA

Fascin

55K-2

1:50 RT1 h

MWO-CB

Dako Corp, Carpinteria, CA, USA

CK14

LL002

1:50 RT1 h

MWO-CB

NeoMarkers, Fremont, CA, USA

CK4

6B10

1:50 4°C overnight

MWO-CB

Novacastra, Newcastle upon Tyne, UK

Annexin 1

29

1:100 RT1 h

MWO-CB

BD Transduction Laboratories, Lexington, KY

Laminin-5γ2

D4B5

1:50 RT1 h

Protease XXIV

Chemicon, Temecula, CA, USA

SPARC

15G12

1:50 RT1 h

MWO-CB

Novacastra, Newcastle upon Tyne, UK

  1. RT, room temperature
  2. MWO-CB, microwave oven heating in citrate buffer.