Figure 1

Representative photographs of immunohistochemical features of the proteins in different lesions. The proteins of interest are designated in rows, and the esophageal histological categories, normal esophageal mucosa (A) and various histological categories of lesions, i.e., mild DYS (B), severe DYS (C) and ESCC (D), are designated in columns. A1-D1, strongly positive expression of Fas in normal epithelia, but negative in mild, severe DYS and ESCC. A2-D2, negative expression of FADD in normal epithelia, but strongly positive in mild, severe DYS and ESCC. A3-D3, positive expression of fascin only in basal cells of normal epithelia, and strongly positive in mild, severe DYS and ESCC.A4-D4, positive expression of CK14 only in basal cells of normal epithelia, negative in mild DYS, but strongly positive in severe DYS and ESCC. A5-D5, negative expression of laminin-5γ2 in cytoplasm of normal epithelia and mild, severe DYS with positive expression only in basement membrane, but strongly positive in cytoplasm of ESCC, especially at the tumor-stroma interface. Moreover, positive expression of laminin-5γ2 can be seen in the budding tumor cells of the microinvasive cancer (E). A6-D6, negative expression of SPARC in the epithelia and stroma in normal mucosa and mild, severe DYS, but prominent SPARC expression by stromal fibroblasts in a background of negative cancer cell reactivity in ESCC. Moreover, SPARC expression can be seen in the cytoplasm of the juxta-stromal tumor cells in a few cases (F). (original magnification, ×200)