Figure 3

Inhibition of anti-CD3/anti-CD28-induced proliferation of peripheral blood T-cells by recombinant LMP1 and galectin 9. Proliferation was measured by [³H] thymidine incorporation after a 3-day culture of PBMCs stimulated by anti-CD3/anti-CD28 beads. Recombinant LMP1dTM1 is deleted of residues 24–78. In the experiment depicted in the bottom panel, LMP1 and galectin 9 were used at 10 ng/ml (0.17 nM) and 0.5 μg/ml (14 nM) respectively whereas in the control experiment, PBMCs were stimulated by CD3/CD28 cross-linking in the absence of additional reagents. Each of these experiments were performed at least 3 times using PBMCs from at least 2 donors.