Figure 6From: Gefitinib (IRESSA) sensitive lung cancer cell lines show phosphorylation of Akt without ligand stimulationFluorescence in situ hybridization (FISH) analyses of lung cancer cell lines, and EGFR mutations and expression. EGFR gene copy numbers were counted and were classified into six FISH strata [9,19]. EGFR gene amplification was detected in PC9 and PC14 cells. In the ABC1 cell line, the EGFR gene copy number was five in over 40% of cells (high polysomy:FISH negative); in the PC7 and LCKJ cell lines, the EGFR gene copy number was three in over 40% of cells (high trisomy:FISH negative).Back to article page