Figure 1

Mouse whole genome CGH microarray data from MIN-Os and tumors. A) Summary of array CGH data by MIN-O lines. Agilent mouse whole genome CGH microarray was used for genomic DNA from different MIN-O and tumor pairs from 4w-4, 4w-11, 8w-B and 8w-D MIN-O mice. The graphs were generated with a smoothing average of 50 MB. Each Pair of MIN-O and corresponding tumor from the same animal is represented with the same color (n = 4 for 4w-4, n = 3 each for 4w-11, 8w-B and 8w-D). 4w-4 and 8w-B samples have multiple whole chromosome gains. 4w-11 and 8w-D samples are relatively genetically stable. B-E) Frequency of whole chromosome gains found in MIN-Os and tumors by array CGH. B) Distribution of whole chromosome gains in all MIN-Os studied with array CGH (n = 14). The most frequent gain is found on chromosome 2, followed by chromosome 1 and 11. C) Distribution of chromosome gains in all tumor samples studied with array CGH (n = 14). Tumors have the same whole chromosome gains found in MIN-Os with a few additional gains. D) Frequency of whole chromosome gain events associated with MIN-O to tumor transition. Whole chromosome gains in tumors that are not found in the corresponding MIN-O samples are presumed to be acquired during the transition from MIN-O to tumor. E) Frequency of chromosome gains in 8w-B and 4w-4 samples (n = 8 for MIN-Os and tumors each). The majority of whole chromosome gains were found in the 8w-B and 4w-4 MIN-O lines.