RT-PCR assessment of MART1, TACC1 , and WT1 mRNA expression in benign and neoplastic human prostate carcinoma samples. - Lanes 1 and 2: negative control (with H2O instead of cDNA), a positive control for each cDNA studied (cDNA from PC3 cell line and from LNCAP cell line for WT1 and TACC1 respectively, and a human metastatic melanoma sample for MART1. - Lanes 3 and 4: n°1 paired benign and malignant (pT2) tissue samples. - Lanes 5 and 6: n°2 paired benign and malignant (pT2) tissue samples. - Lanes 7–10: distinct pT2 carcinoma samples. - Lanes 11–15: distinct pT3 carcinoma samples (13 and 15 are M+ carcinomas). - Lanes 16–20: distinct HR carcinomas samples. 1st line: WT1 mRNA is detected as a 706 bp PCR product (variant) in 2/6 pT2 carcinoma samples, and as a 851 bp product (wild-type isoform) in 1/2 N1/M1 and 5/5 HR carcinoma samples. No WT1 mRNA is present in benign prostate tissue samples. 2nd line: MART1 mRNA is expressed in only 1/6 pT2 carcinoma sample, but neither in any advanced (pT3 and/or N1/M1) stage nor HR carcinoma samples. 3rd line: TACC1 mRNA is more strongly expressed in both pT2 and pT3 carcinoma than in benign tissue samples. TACC1 mRNA is also expressed in 3/5 HR samples. 4th line: α-actin mRNA amplification is used as a quality control for mRNA extraction and RT-PCR reaction.