Figure 1From: A novel copper complex induces ROS generation in doxorubicin resistant Ehrlich ascitis carcinoma cells and increases activity of antioxidant enzymes in vital organs in vivo(A) Effect of exogenous H2O2 on MRP1 expression. EAC/Dox cells were incubated overnight in presence of 50 μM H2O2. Surface MRP1 expression was studied by flow cytometry and confocal microscopy. Representative data of 5 independent experiments is presented. (B) ROS generation by EAC/Dox cells ex vivo 24 h following in vivo CuNG treatment. CuNG treatment given after 7 days following inoculation with EAC/Dox cells. Results are compared with ex vivo ROS generation by EAC cells derived from EAC/S and EAC/Dox bearing mice. EAC/Dox cells following in vivo CuNG treatment and EAC/S cells generated significantly higher levels of ROS compared to EAC/Dox cells from untreated animals (*, P < 0.001 and **, P < 0.005). Results are presented as mean ± S.D. of 3 independent experiments. Each experiment was done with 5 animals in each experimental group. (C) Confocal microscopy of the effect of CuNG on MRP1 expression in EAC/Dox cells. EAC/Dox cells expressed higher levels of MRP1 than EAC/S cells and CuNG treatment reduced MRP1 expression. Results presented are representative of 3 independent experiments.Back to article page