Rho-like small GTPase activation in Rat-1 cells expressing t(9;22) translocation products. A. Expression of the transgenes in Rat-1 cells was controlled by Western blot analysis using α-ABL and α-BCR antibodies as indicated. B. The indicated activated small GTPase was pulled down and revealed by the specific antibody. Rac*, cdc42*, Rho* – activated forms; total Rac, cdc42, Rho – total amount revealed in the cell lysates used for the pull down. One representative experiment of three performed is given.