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Table 1 The monoclonal primary mouse antibodies used in this study.

From: Effects of estradiol and medroxyprogesterone acetate on morphology, proliferation and apoptosis of human breast tissue in organ cultures

Primary antibody Company Antibody dilution Staining pattern
Actin, Smooth Muscle Ab-1 NeoMarkers, Fremont, USA 1:400 Cytoplasmic
anti-Cytokeratin 8&18 Zymed® Laboratories Inc, South San Francisco, CA 1:250 Cytoplasmic
anti-Human Estrogen Receptor – alpha DakoCytomation, Glostrup, Denmark 1:2000 Nuclear
Anti-PCNA Zymed® Laboratories Inc, South San Francisco, CA 1:200 Nuclear
Estrogen Receptor – beta Novocastra, Newcastle Upon Tyne, UK 1:800 Nuclear
Keratin 14 Ab-1 NeoMarkers, Fremont, USA 1:400 Cytoplasmic
Ki67 Antigen Novocastra, Newcastle Upon Tyne, UK 1:100 Nuclear
Progesterone Receptor (A/B Forms) Novocastra, Newcastle Upon Tyne, UK 1:100 Nuclear
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BMC Cancer

ISSN: 1471-2407

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