Table 1 The monoclonal primary mouse antibodies used in this study.
Primary antibody | Company | Antibody dilution | Staining pattern |
|---|---|---|---|
Actin, Smooth Muscle Ab-1 | NeoMarkers, Fremont, USA | 1:400 | Cytoplasmic |
anti-Cytokeratin 8&18 | Zymed® Laboratories Inc, South San Francisco, CA | 1:250 | Cytoplasmic |
anti-Human Estrogen Receptor – alpha | DakoCytomation, Glostrup, Denmark | 1:2000 | Nuclear |
Anti-PCNA | Zymed® Laboratories Inc, South San Francisco, CA | 1:200 | Nuclear |
Estrogen Receptor – beta | Novocastra, Newcastle Upon Tyne, UK | 1:800 | Nuclear |
Keratin 14 Ab-1 | NeoMarkers, Fremont, USA | 1:400 | Cytoplasmic |
Ki67 Antigen | Novocastra, Newcastle Upon Tyne, UK | 1:100 | Nuclear |
Progesterone Receptor (A/B Forms) | Novocastra, Newcastle Upon Tyne, UK | 1:100 | Nuclear |