Effects of estradiol and medroxyprogesterone acetate on morphology, proliferation and apoptosis of human breast tissue in organ cultures

Table 1 The monoclonal primary mouse antibodies used in this study.

Primary antibody	Company	Antibody dilution	Staining pattern
Actin, Smooth Muscle Ab-1	NeoMarkers, Fremont, USA	1:400	Cytoplasmic
anti-Cytokeratin 8&18	Zymed® Laboratories Inc, South San Francisco, CA	1:250	Cytoplasmic
anti-Human Estrogen Receptor – alpha	DakoCytomation, Glostrup, Denmark	1:2000	Nuclear
Anti-PCNA	Zymed® Laboratories Inc, South San Francisco, CA	1:200	Nuclear
Estrogen Receptor – beta	Novocastra, Newcastle Upon Tyne, UK	1:800	Nuclear
Keratin 14 Ab-1	NeoMarkers, Fremont, USA	1:400	Cytoplasmic
Ki67 Antigen	Novocastra, Newcastle Upon Tyne, UK	1:100	Nuclear
Progesterone Receptor (A/B Forms)	Novocastra, Newcastle Upon Tyne, UK	1:100	Nuclear

ISSN: 1471-2407