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Table 2 Cyclin D1a and cyclin D1b mRNA expression in MCL and MM samples

From: Relevance of cyclin D1b expression and CCND1polymorphism in the pathogenesis of multiple myeloma and mantle cell lymphoma

Sample ΔCta ΔΔCta Na ΔCtb ΔΔCtb Nb
MCL       
JeKo-1* 6.5 0 1 12.7 0 1
GRANTA-519 12.5 6.0 0.0156 19.0 6.3 0.0127
HBL-2 7.8 1.3 0.4061 14.5 1.8 0.2871
NCEB-1 10.9 4.4 0.0474 19.7 7.0 0.0078
Pt 1 10.4 3.9 0.0670 19.3 6.6 0.0103
Pt 2 11.3 4.8 0.0359 16.4 6.7 0.0096
Pt 3 10.1 5.6 0.0206 nc nc nc
Pt 4 12.9 6.4 0.0118 18.2 8.5 0.0028
Pt 5 9.2 2.7 0.1539 15.5 5.8 0.0179
Pt 6 8.8 2.3 0.2030 15.0 5.3 0.0254
Pt 7 11.4 4.9 0.0335 16.9 7.2 0.0068
Pt 8 9.1 2.6 0.1650 14.3 4.6 0.0412
Pt 9 12.2 5.7 0.0192 17.0 7.3 0.0063
MM       
U266* 10.0 0 1 18.8 0 1
Karpas 620 13.9 3.9 0.0670 20.8 2.0 0.2500
RPMI 8226 17.1 7.1 0.0073 23.9 5.1 0.0291
NCI-H929 15.9 5.9 0.0167 23.7 4.9 0.0335
OPM-2 nc nc nc nc nc nc
Pt 1 12.3 2.3 0.2030 18.5 -0.3 1.2311
Pt 2 18.2 8.2 0.0034 27.7 8.9 0.0021
Pt 3 15.0 5.0 0.0312 27.1 8.3 0.0032
  1. The quantitation of cyclin D1a and cyclin D1b mRNA compared to 18S rRNA was done using the comparative threshold (Ct) method with the ABI PRISM 7000 SDS software. Triplicate experiments were done for each sample; for each sample the average Ct value for the internal standard was subtracted from the average Ct value for cyclin D1a or cyclin D1b to yield ΔCta and ΔCtb. ΔCt obtained from the calibrator cell lines (* JeKo-1 for MCL samples and U266 for MM samples) was subtracted from each ΔCta or ΔCtb to give the ΔΔCt. The relative amount of cyclin D1a and cyclin D1b compared to the calibrator was calculated by the formula N = 2-ΔΔCt. No cyclin D1b was detected in MCL patient 3 (Ctb>50); no cyclin D1a or b was detected in OPM-2 MM cell line (Cta>50 and Ctb>50). nc, not calculated; Pt, patient.