|
Sample
|
ΔCta
|
ΔΔCta
|
Na
|
ΔCtb
|
ΔΔCtb
|
Nb
|
|---|
|
MCL
| | | | | | |
|
JeKo-1*
|
6.5
|
0
|
1
|
12.7
|
0
|
1
|
|
GRANTA-519
|
12.5
|
6.0
|
0.0156
|
19.0
|
6.3
|
0.0127
|
|
HBL-2
|
7.8
|
1.3
|
0.4061
|
14.5
|
1.8
|
0.2871
|
|
NCEB-1
|
10.9
|
4.4
|
0.0474
|
19.7
|
7.0
|
0.0078
|
|
Pt 1
|
10.4
|
3.9
|
0.0670
|
19.3
|
6.6
|
0.0103
|
|
Pt 2
|
11.3
|
4.8
|
0.0359
|
16.4
|
6.7
|
0.0096
|
|
Pt 3
|
10.1
|
5.6
|
0.0206
|
nc
|
nc
|
nc
|
|
Pt 4
|
12.9
|
6.4
|
0.0118
|
18.2
|
8.5
|
0.0028
|
|
Pt 5
|
9.2
|
2.7
|
0.1539
|
15.5
|
5.8
|
0.0179
|
|
Pt 6
|
8.8
|
2.3
|
0.2030
|
15.0
|
5.3
|
0.0254
|
|
Pt 7
|
11.4
|
4.9
|
0.0335
|
16.9
|
7.2
|
0.0068
|
|
Pt 8
|
9.1
|
2.6
|
0.1650
|
14.3
|
4.6
|
0.0412
|
|
Pt 9
|
12.2
|
5.7
|
0.0192
|
17.0
|
7.3
|
0.0063
|
|
MM
| | | | | | |
|
U266*
|
10.0
|
0
|
1
|
18.8
|
0
|
1
|
|
Karpas 620
|
13.9
|
3.9
|
0.0670
|
20.8
|
2.0
|
0.2500
|
|
RPMI 8226
|
17.1
|
7.1
|
0.0073
|
23.9
|
5.1
|
0.0291
|
|
NCI-H929
|
15.9
|
5.9
|
0.0167
|
23.7
|
4.9
|
0.0335
|
|
OPM-2
|
nc
|
nc
|
nc
|
nc
|
nc
|
nc
|
|
Pt 1
|
12.3
|
2.3
|
0.2030
|
18.5
|
-0.3
|
1.2311
|
|
Pt 2
|
18.2
|
8.2
|
0.0034
|
27.7
|
8.9
|
0.0021
|
|
Pt 3
|
15.0
|
5.0
|
0.0312
|
27.1
|
8.3
|
0.0032
|
- The quantitation of cyclin D1a and cyclin D1b mRNA compared to 18S rRNA was done using the comparative threshold (Ct) method with the ABI PRISM 7000 SDS software. Triplicate experiments were done for each sample; for each sample the average Ct value for the internal standard was subtracted from the average Ct value for cyclin D1a or cyclin D1b to yield ΔCta and ΔCtb. ΔCt obtained from the calibrator cell lines (* JeKo-1 for MCL samples and U266 for MM samples) was subtracted from each ΔCta or ΔCtb to give the ΔΔCt. The relative amount of cyclin D1a and cyclin D1b compared to the calibrator was calculated by the formula N = 2-ΔΔCt. No cyclin D1b was detected in MCL patient 3 (Ctb>50); no cyclin D1a or b was detected in OPM-2 MM cell line (Cta>50 and Ctb>50). nc, not calculated; Pt, patient.
