The E1A-HD2 virus shows increased toxicity on cells with activated Wnt signaling. A. CPE assays. Cells were infected with 10-fold dilutions of virus starting from 100 pfu/cell in HeLa, Hct116 and HT29 cells, and starting from 10 pfu/cell in ISREC-O1 and SW480 cells. B. MTT assays in the same cell lines. Dotted line, parental virus. Solid line, E1A-HD2 virus. C. The change in EC50 calculated from the data in B.