Figure 3

E1A-HD2 expression promotes nuclear localization of β-catenin. SW480 cells were mock infected (A-D), infected with the parental virus (E-H), or infected with the E1A-HD2 virus (I-L), and stained for β-catenin (A, E, I) or E1A (C, G, K). DAPI staining was used to identify the nuclei (B, D, F, H, J, L). The primary antibody was omitted in M to verify the specificity of the secondary antibody used to detect β-catenin.