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Figure 4 | BMC Cancer

Figure 4

From: Targeting of mutant hogg1in mammalian mitochondria and nucleus: effect on cellular survival upon oxidative stress

Figure 4

Real Time PCR Amplification Curves. Representative real-time PCR amplification curves generated for nuclear β-actin gene (Fig. 4A.), mitochondrial D-Loop (Fig. 4B.) and Cox I (Fig. 4C.) with and without wild type MTS-hOGG1 after treatment with 400 μM H2O2. Each experiment was performed in triplicate and is shown by overlapping amplification curves. ΔRn = (Rn+) - (Rn-), where Rn+ is the fluorescence emission intensity of reporter/emission intensity of quencher at any time point, and Rn- is the initial emission intensity of reporter/emission intensity of quencher in the same reaction vessel before PCR amplification is initiated.

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