Figure 3

Cell Survival of hOGG1 Regulated Cells. HeLa cells transfected with indicated plasmids were grown in regular growth media, for 72 h post transfection. The cells were plated at a density such that they reached a 70% confluence on the day of the treatment. The cells were then treated with 400 μM H₂O₂ (A) for 2 h and for 1 h with 4NQO (B) in serum free media, and allowed to recover in normal growth medium for 16 h. Cellular survival was assessed using the MTT cell proliferation assay kit. Error bars represent standard deviation of four points. Nuc-hOGG1 (nuclear targeted hOGG1); MTS-hOGG1 (mitochondrially-targeted hOGG1), Nuc-mutant-hogg1 (nuclear targeted mutant hogg1), MTS-mutant-hogg1 (mitochondrially-targeted mutant hogg1), Vector (empty vector). p < 0.05 when the data from Nuc-hOGG1, MTS-hOGG1 and Nuc-Mutant-hogg1 transfectants was compared with vector-only cells using Student's t test.