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Figure 2 | BMC Cancer

Figure 2

From: Targeting of mutant hogg1in mammalian mitochondria and nucleus: effect on cellular survival upon oxidative stress

Figure 2

Expression of hOGG1 targeted to mitochondria. HeLa cells were transfected with empty vector, MTS-hOGG1, MTS-mutant-hogg1, Nuc-hOGG1, and Nuc-mutant-hogg1. Mitochondrial, nuclear and total cellular extracts were isolated and analyzed by Western blot analysis using anti-OGG1 antiserum. Twenty microgram of mitochondrial, nuclear and total cellular extract for each indicated transfection was loaded into each lane (Figure 2A. & B.). Protein extracts in each lane are as indicated. Immunodetection of Lamin B and COX II was done to assure that the transfected proteins were in nucleus and mitochondria respectively.

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