Figure 1

Sequence of hogg1 Mutant. hOGG1 (wild type) and mutant-hogg1 (mutant) were transfected in HeLa cells. 72 h post transfection, total RNA was isolated and RT-PCR was performed using Superscript II (Invitrogen, Carlsbad, CA) as per manufacturer's instructions. Automated DNA Sequencing results indicated that cells transfected with mutant-hogg1 harbored mutant hogg1, where CGA is changed to CAA at codon 229. Arrow indicates mutated position (G to A).