Translating microarray data for diagnostic testing in childhood leukaemia

Table 2 Top 30 probe sets identified by RMA/RF that are required for accurate classification.

Subgroup	Probe ID	Gene	Rank RMA/RF	Rank Ross et al ^†	Expression above/below mean	Fold change
BCR-ABL	201906_s_at	CTDSPL	1	17	Above	2.5
	209365_s_at*	ECM1	2	3	Above	5.0
	210830_s_at*	PON2	3	2	Above	5.7
	202123_s_at	ABL1	4	76	Above	2.0
	222154_s_at	DNAPTP6	5	13	Above	6.9
E2A-PBX1	212148_at*	PBX1	1	4	Above	29.6
	221113_s_at	WNT16	2	18	Above	6.2
	205253_at*	PBX1	3	3	Above	36.1
	230306_at^•	MGC10485	4	11	Above	5.9
	225483_at^•	MGC10485	5	8	Above	8.2
Hyperdipl.>50	200659_s_at	PHB	1	-	Above	2.8
	216071_x_at*	TNRC11	2	1	Above	1.7
	208598_s_at	UREB1	3	12	Above	1.6
	226875_at^•	DOCK11	4	35	Above	2.3
	200057_s_at	NONO	5	-	Above	1.5
MLL	226939_at*^•	CPEB2	1	1	Above	5
	219463_at*	C20orf103	2	2	Above	15.2
	204069_at*	MEIS1	3	3	Above	10
	218847_at	IMP-2	4	24	Above	7
	201152_s_at	MBNL1	5	23	Above	2.2
T-ALL	213539_at	CD3D	1	17	Above	28.3
	227646_at^•	EBF	2	23	Below	24.6
	217147_s_at	TRIM	3	20	Above	10.1
	229487_at^•	EBF	4	24	Below	20.4
	50221_at	TFEB	5	-	Below	2.2
TEL-AML1	206231_at	KCNN1	1	15	Above	3.2
	241505_at*^•	EST	2	4	Above	5.8
	206033_s_at	DSC3	3	12	Above	8.8
	214110_s_at	EST	4	-	Above	2.2
	206032_at	DSC3	5	11	Above	4.8

† The ranking refers to the top 100 subgroup-discriminating probe sets identified by Ross et al. using the parallel analysis format.
* Probe sets/genes identified as the top 5 subgroup-discriminators in both analyses, RMA/RF and Ross et al. (parallel format).
• Probe sets that are represented on the HG-U133B array.